Figure 2: TPL downregulates HER2 expression and PI3K/Akt activity in SKOV-3 cells. (a) SKOV-3 cells were treated with various doses of TPL or PBS for 48 h (left panel) and 50 nM of TPL for the indicated time (right panel). HER2 and actin protein levels were determined by western blotting. Densitometric analysis of the percentage of decrease was determined using Adobe Photoshop software (b). HER2 expression was downregulated by TPL in SKOV-3 cell line in a dose- and time-dependent manner. (c) Downregulation of HER2 and phospho-PI3K/Akt by TPL. SKOV-3 cells were treated with the indicated concentration of TPL for 48 h. Western blot analysis of cell lysates was performed with antibodies recognizing HER2, phospho-HER2-Y1248 (p-HER2), p85 PI3K, phospho-Y p85 PI3K (p-PI3K), Akt, phospho-Akt-S473 (p-Akt), extracellular signal-regulated kinase (ERK), phospho-extracellular signal-regulated kinase-T202/Y204 (p-ERK), and actin. The densitometric analysis was performed as previously mentioned and is shown in (d). The data are presented as the mean ± standard error from three independent experiments. and versus the vehicle-treated control group.