Figure 2: Effect of berberine on human preadipocyte proliferation. Cells were cultured in growth medium with different concentrations of berberine for 1, 2, and 3 days. At each culture time point, proliferation capacity was determined by MTT assay. Values are expressed as percentage of the untreated controls and represent the mean ± SEM of the three separate experiments in eight replicates. * , compared to control at each time point.