Effects of Bee Venom on Glutamate-Induced Toxicity in Neuronal and Glial Cells
Figure 4
Glutamate-mediated changes in protein expression in BV2 cells and effects of BV pretreatment. BV2 cells were seeded in 60 mm plates (2 × 106/plate). (a) After a 24-h incubation, BV2 cells were treated for the indicated incubation time with 5 mM glutamate. Cell lysates were analyzed with western blotting with phospho-JNK or JNK antibodies. (b) BV2 cells were treated with 2.5 μg/mL BV for 30 min prior to stimulation with 5 mM glutamate for 30 min. (c) After a 24-h incubation, BV2 cells were treated for the indicated incubation time with 5 mM glutamate. Cell lysates were analyzed with western blotting with phospho-Akt or Akt antibodies. The activation of Akt was calculated based on the relative phospho-/nonphosphor- ratio as indicated. (d) BV2 cells were treated with 2.5 μg/mL BV for 30 min prior to stimulation with 5 mM glutamate for 30 min. Western blot analysis was performed with phospho-Akt or total Akt antibodies and evaluated based on activated Akt/Akt ratio. Levels of tubulin were measured to control for protein loading. * indicates P < 0.05 compared to untreated cells, and ## indicates P < 0.005 compared to the corresponding control.
