Xiaoyaosan Decoction Regulates Changes in Neuropeptide Y and Leptin Receptor in the Rat Arcuate Nucleus after Chronic Immobilization Stress

Figure 5

In situ hybridization for ob-R mRNAin the ARC. (A–D) Representative images of ob-R mRNA in ARC. (A) The control group. (B) The 7-day stress group. (C) The 21-day stress group. (D) The Xiaoyaosan-treated group. Scale bars = 100 μm for A (I) and 10 μm for other images. IOD of ob-R mRNA labeling and cells of ob-R mRNA labeling were measured with Image Pro Plus. Each bar represent the mean values and vertical bars represent SEM. ^⋇𝑃<0.05 or ^⋇⋇𝑃<0.01 as compared with the control group; ^Δ𝑃<0.05 as compared with the 7-day stress group.