Isolation and Identification of Endophytic Fungi from Actinidia macrosperma and Investigation of Their Bioactivities
Table 1
Identification and biological activity of endophytic fungi isolated from A. macrosperma a.
GenBank acc. no.
Endophytic fungi code
Identified speciesc
% Similarity
Cytotoxic activity LC50 (μg/mL)
Antitumor activity LC50 (μg/mL)
HepG2
MCF7
A549
SGC-7901
HeLa
JN596334
AM01
Acremonium furcatum (HQ637291)
100
325.72 (198.00~792.90)
87.75 (45.86~165.40)
88.70 (38.94~133.34)
85.77 (54.82~176.32)
>100
99.08 (25.62~383.20)
JN596335
AM02
Acremonium furcatum (JF311939)
100
206.31 (126.20~437.21)
81.05 (56.32~120.06)
76.29 (28.74~165.30)
70.98 (36.26~98.87)
92.56 (53.24~322.14)
71.83 (57.64~89.51)
JN596336
AM03
Acremonium furcatum (DQ865092)
99
262.94 (184.72~421.58)
90.63 (65.52~142.30)
88.65 (52.72~128.80)
84.80 (53.73~128.73)
94.07 (79.16~111.80)
83.92 (62.20~131.03)
JN596337
AM04
Acremonium furcatum (DQ865092)
100
291.30 (236.63~358.67)
93.60 (85.27~127.54)
91.98 (55.45~167.76)
89.15 (44.08~122.66)
97.74 (63.22~143.24)
94.9 (65.72~157.74)
JN596338
AM05
Cylindrocarpon pauciseptatum (HQ441248)
98
>1000
>100
>100
>100
>100
>100
JN596339
AM06
Cylindrocarpon pauciseptatum (AB369414)
100
>1000
>100
>100
>100
>100
>100
JN596340
AM07
Trichoderma citrinoviride (HQ608144)
100
4.86 (2.95~7.55)
3.13 (2.26~5.72)
3.07 (2.82~6.01)
2.00 (1.89~5.43)
3.44 (2.08~5.63)
2.88 (2.14~6.37)
JN596341
AM08
Trichoderma citrinoviride (HQ608144)
99
35.88 (34.02~37.84)
24.63 (12.34~50.77)
22.10 (15.85~31.17)
16.22 (10.45~27.31)
23.66 (20.22~44.38)
22.08 (10.28~53.24)
JN596342
AM09
Trichoderma citrinoviride (AF414343)
98
21.20 (11.10~32.20)
11.03 (4.29~19.72)
10.64 (7.70~20.10)
8.83 (4.42~14.09)
12.57 (9.41~18.09)
10.11 (5.82~24.40)
JN596343
AM10
Paecilomyces marquandii (FR799470)
100
>1000
>100
>100
>100
>100
>100
JN596344
AM11
Chaetomium globosum (JF826003)
99
7.71 (7.30~8.12)
3.87 (2.96~5.45)
3.92 (2.74~7.14)
3.14 (2.27~6.62)
4.05 (3.01~6.29)
3.86 (2.87~7.33)
JN596345
AM12
Ascomycete Incertae sedis sp. (HQ623458)
100
124.54 (67.51~183.57)
53.77 (21.10~82.23)
48.63 (23.11~61.76)
46.92 (21.10~76.50)
74.77 (64.83~86.23)
45.47 (18.89~67.71)
JN596346
AM13
Ascomycete Incertae sedis sp. (AM944361)
99
228.32 (170.54~311.08)
75.70 (69.05 ~82.99)
75.22 (69.59~81.30)
58.21 (39.79~85.15)
85.39 (35.62~119.04)
69.14 (23.66~108.74)
JN596347
AM14
Ascomycete Incertae sedis sp. (HQ623458)
99
179.09 (131.23~243.95)
46.64 (31.10~62.29)
50.33 (42.20~60.02)
47.47 (20.03~59.99)
68.10 (29.74~153.40)
44.43 (30.07~52.90)
JN596348
AM15
Ascomycete Incertae sedis sp. (AF413049)
95
490.79 (258.30~682.34)
>100
>100
89.35 (63.32~112.31)
>100
98.26 (55.40~182.31)
JN596349
AM16
Ascomycete Incertae sedis sp. (AM944361)
97
61.57 (36.53~103.61)
31.83 (10.98~53.26)
35.88 (34.02~37.84)
28.93 (20.15~33.29)
39.04 (33.19 ~45.92)
33.36 (26.41~42.15)
JN596350
AM17
Ascomycete Incertae sdis sp. (GU067747)
100
14.88 (14.05~15.81)
7.90 (5.33~20.15)
8.59 (5.27~16.21)
6.79 (4.63~12.28)
10.04 (6.29~14.95)
8.53 (6.22~19.65)
/
Podophyllo-toxinb
/
/
2.72 (2.38~3.50)
0.94 (0.66~2.03)
1.02 (0.75~1.99)
0.75 (0.58~1.77)
1.37 (0.98~2.23)
0.84 (0.62~1.52)
a All determinations were done in triplicate, 95% confidence limits in parentheses. No mortality with negative control group (1% DMSO).
b Positive control group; c Closest identified relatives were shown in brackets (by BLAST search).
