Inhibition of Hypoxia Inducible Factor Alpha and Astrocyte-Elevated Gene-1 Mediates Cryptotanshinone Exerted Antitumor Activity in Hypoxic PC-3 Cells
Figure 3
HIF-1α siRNA transfection attenuates AEG-1 expression and the viability of PC-3 cells under hypoxia, but DMOG enhances the expression of HIF-1α and AEG-1 in hypoxic PC-3 cells. (a) Effect of HIF-1α siRNA transfection or DMOG on HIF-1α stability and AEG-1 expression in hypoxic PC-3 cells. Cells were transfected with either control siRNA vector, HIF-1α siRNA, or AEG-1 siRNA and after 24 h were incubated in hypoxia for an additional 24 h. For DMOG treatment, Cells treated with DMOG for 24 hours under hypoxia were fixed with 10% formalin for immunocytochemistry fluorescence staining. Detection of HIF-1α (red) and AEG-1 (green) in PC-3 cells. Nuclei (blue) were counterstained using DAPI. (b) Effect of HIF-1α siRNA transfection on the viability of hypoxic PC-3 cells. Cells transfected with either non-target siRNA, HIF-1α siRNA were incubated under hypoxia for 48 h and the viability of the cells was evaluated by using BrdU assay. (c) Effect of cryptotanshinone on cell death in PC-3 cells. Cells were treated with cryptotanshinone (5, 10 μM) for 48 h. (d) HIF-1α inhibition is necessary for CK-induced apoptotic cell death in PC-3 cells. PC-3 cells were transfected with either siRNA control or siRNA HIF-1α for 24 h and exposed to 10 μM cryptotanshinone for 48 h.
