Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis

<table>HPTLC chromatogram of sphingolipid standards. Sphingomyelin, dihydrosphingomyelin, ceramide-1-phosphate, sphingosine-1-phosphate, ceramide, and sphingosine standards were spotted on the HPTLC plate, developed in chloroform/methanol/2 M aqueous ammonia (65 : 25 : 4, v/v/v) for 1 h, and visualized with 10% sulfuric acid. </table>

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Figure 2

Figure 2: Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis 