Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis
Figure 5
Optimization of enzymatic hydrolysis. Sphingomyelin and dihydrosphingomyelin standards were incubated (a) for varying periods of time (0.5, 1, 2, 4, 8, 12, 16, 20, and 24 h), (b) at the range of concentrations (10–1,000 pmol), and (c) 1,000 pmol sphingomyelin standard was simultaneously incubated with SMase and SCDase at the range of enzyme activities (5–200 μunits) for 12 h, followed by HPLC analysis. Values are expressed as the mean ± SD ().
