Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis

Table 1

Intra- and inter-day CVs for the sphingomyelin standard.

Sphingomyelin
	Added (pmol)	Measured (pmol)	CV^a (%)
Intra-day (𝑛=10)	100	98.18 ± 2.04	2.08
	200	189.05 ± 2.68	1.42
	500	483.68 ± 5.87	1.22
	1,000	980.51 ± 6.95	0.71
Inter-day (𝑛=10)	100	96.49 ± 2.14	2.22
	200	191.46 ± 2.55	1.33
	500	478.52 ± 5.57	1.16
	1,000	957.13 ± 6.68	0.70

^aCoefficient of variation.