Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis

Table 2

Intra- and inter-day CVs and accuracy for the sphingomyelin in plasma and liver from ICR mice and 3T3-L1 cells.

Samples	Added	Intra-day (𝑛=10)			Inter-day (𝑛=10)
		Measured^a (pmol)	CV^b (%)	Accuracy (%)	Measured^a (pmol)	CV^b (%)	Accuracy (%)
Mouse plasma (μL)	1	405.51±0.25	1.52	99.54	403.05±0.20	1.17	98.93
	5	2004.22±0.14	0.85	98.39	2018.23±1.55	0.21	99.07
	10	3984.02±3.15	0.80	97.80	4001.58±6.50	2.50	98.21
	20	8320.05±9.26	8.43	102.11	8247.09±8.90	6.02	101.22
Mouse liver (μg protein)	1	23.01±0.02	0.71	103.37	22.05±0.50	0.54	99.06
	2	44.50±0.27	1.16	99.96	45.54±2.15	1.27	102.30
	5	115.25±3.90	0.98	103.55	109.46±5.30	1.15	98.37
	10	219.19±9.00	2.54	98.47	216.41±7.25	1.72	97.22
3T3-L1 cells (μg protein)	1	60.05±0.15	0.10	99.92	62.00±0.20	0.25	103.16
	2	121.47±2.21	1.14	101.06	124.02±5.44	2.10	103.18
	5	314.10±8.06	1.21	104.53	308.95±9.75	2.04	102.81
	10	587.12±8.25	0.78	97.67	605.46±6.15	1.45	100.74

^aValues are expressed as the mean ± S.D. in μM for mouse plasma and pmol/μg protein for mouse tissues and cultured cells.
^bCoefficient of variation.