Demethoxycurcumin Retards Cell Growth and Induces Apoptosis in Human Brain Malignant Glioma GBM 8401 Cells
Figure 6
DMC inhibits nuclear NF-κB transcription factor activity in GBM 8401 cells. (a) The NoShift II transcription factor assay kit was used to identify the activity of NF-κB transcription factor in the cells after exposure to DMC followed by examination with microplate luminometer. (b) Diminished NF-κB activity in DMC-treated GBM8401 cells. The cells were examined for their NF-κB activity 6 hours after DMC stimulation by confocal microscopy of NF-κB subunit p50/52 localization. The cells were stained for p50/52 (red). DAPI (blue) indicates nucleus, where an active form of NF-κB subunit p50/52 is found. All data were reported as the means (±SEM) of at least three separate experiments. Statistical analysis was as cited above.
