Green Tea Attenuates Oxidative Stress and Downregulates the Expression of Angiotensin II AT1 Receptor in Renal and Hepatic Tissues of Streptozotocin-Induced Diabetic Rats
Figure 6
Immunohistochemical localization of AT1 receptor expression in the outer medulla of the kidney of normal control (a), diabetic control (b), and green-tea-treated diabetic (c) rats. Renal sections were treated with the polyclonal anti-AT1 receptor antibody and counterstained with hematoxylin as detailed in Materials and Methods. In (a), the labeling was evident in vasa recta bundles and, to a lesser extent, the basolateral side of collecting tubules and the apical side of collecting ducts, whereas Henle’s loop segments were not labeled. In (b), the labeling was of relatively low intensity in the vasa recta bundles but was evident in the epithelial lining of collecting tubules and ducts as well as Henle’s loop segments. In (c), labeling with faded intensity was observed in vasa recta bundles, and confined to the basolateral side of collecting tubules, collecting ducts, and Henle’s loop epithelia. No specific labeling was observed in kidney tissue sections labeled with the anti-AT1 antibody preabsorbed with an AT1 octapeptide/BSA complex (d). Images are representative of four different samples for each rat group. A: ascending limb of Henle’s loop, CD: collecting duct, CT: collecting tubule, D: descending limb of Henle’s loop, and VR: vasa recta bundle. , ×100.
