Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane
Figure 2
Arrest of cell cycle progression at G2/M and subG0/G1 in response to SFN treatment. (a) SFN-induced G2/M (SFN 6.25 μM) and G0/G1 (SFN 12.5 and 25 μM) arrest in primary CRC cell lines. The distribution of the cell cycle of CRC cell lines was assessed by flow cytometry after staining with propidium iodide (PI). (b) Results were expressed as a percentage of subG0/G1. All data were reported as the means (±SEM) of at least three separate experiments. Statistical analysis used the t-test, with the significant differences determined at the level of versus SFN 0 μM group, while the symbol on the bar denotes the difference which is statistically significant at as compared to the SFN 6.25 (#) or 12.5 μM (&).
