Furanodiene Induces Endoplasmic Reticulum Stress and Presents Antiproliferative Activities in Lung Cancer Cells
Figure 3
FUR induces ER stress in lung cancer cells. (a) Effect of FUR on the mRNA levels of BIP and CHOP in A549 and 95-D cells. Cells were treated with different concentrations of FUR for 12 h, and mRNA levels were determined by RT-PCR. (b) Effect of FUR on the protein levels of BIP and CHOP in A549 and 95-D cells. Cells were treated with different concentrations of FUR for 12 h or 24 h, and protein levels were determined by Western blot. (c) Immunocytochemical staining was conducted to detect the expression of CHOP in nuclei. Cells were treated with 80 μM FUR for 12 h and the cells were fixed and stained with anti-CHOP antibody (green) and Hoechst 33342 (blue). Bar: 10 μm.
