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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 429320, 12 pages
Research Article

Anti-Inflammatory Activities of Cinnamomum cassia Constituents In Vitro and In Vivo

1School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan
2Department of Health and Nutrition Biotechnology, Asia University, Taichung 413, Taiwan
3School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan
4Department of Nursing, Hsin Sheng College of Medical Care and Management, Taoyuan 325, Taiwan
5Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 250, Taiwan

Received 25 August 2011; Revised 11 January 2012; Accepted 11 January 2012

Academic Editor: Jairo Kenupp Bastos

Copyright © 2012 Jung-Chun Liao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We have investigated the anti-inflammatory effects of Cinnamomum cassia constituents (cinnamic aldehyde, cinnamic alcohol, cinnamic acid, and coumarin) using lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) and carrageenan (Carr)-induced mouse paw edema model. When RAW264.7 macrophages were treated with cinnamic aldehyde together with LPS, a significant concentration-dependent inhibition of nitric oxide (NO), tumor necrosis factor (TNF-α), and prostaglandin E2 (PGE2) levels productions were detected. Western blotting revealed that cinnamic aldehyde blocked protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear transcription factor kappa B (NF-κB), and IκBα, significantly. In the anti-inflammatory test, cinnamic aldehyde decreased the paw edema after Carr administration, and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the paw tissue. We also demonstrated cinnamic aldehyde attenuated the malondialdehyde (MDA) level and myeloperoxidase (MPO) activity in the edema paw after Carr injection. Cinnamic aldehyde decreased the NO, TNF-α, and PGE2 levels on the serum level after Carr injection. Western blotting revealed that cinnamic aldehyde decreased Carr-induced iNOS, COX-2, and NF-κB expressions in the edema paw. These findings demonstrated that cinnamic aldehyde has excellent anti-inflammatory activities and thus has great potential to be used as a source for natural health products.