Neuroprotective Activity of Sibjeondaebo-tang on A Peptide-Induced Damages
Figure 5
Induction of phospho-Erk1/2 in the cerebral cortex. (a) Western blot analysis. After various treatments into mice, cerebral cortical tissues were dissected out and used for western blot analysis. Western analysis for total Erk1/2 protein for the same transferred membrane was used as an internal loading control. 1: normal, 2: A (200 pmol/5 μL), 3: A+galantamine (3 mg/kg), and 4: A+SJDBT extract (400 mg/kg), (b, c). Immunofluorescence staining of brain sections. (b) Brain sections were used for double immunofluorescence staining for NF-200 protein (green) and phospho-Erk1/2 protein (red), and the merged images were shown in the figure. (c) Immunofluorescence view of phospho-Erk1/2 protein signals (red) in NF-200-stained cortical sections (green). Merged view indicates that the area where phospho-Erk1/2 signals are relatively strong is the central zone surrounded by NF-200-stained processes (arrowheads).
