A Novel Herbal Medicine KIOM-MA Exerts an Anti-Inflammatory Effect in LPS-Stimulated RAW 264.7 Macrophage Cells
Figure 2
Effect of KIOM-MA on TNF-α (a) cytokine production and (b) mRNA expression upon LPS stimulation. RAW 264.7 cells were pretreated with KIOM-MA for 30 min before being incubated with LPS for (a) 24 hours and (b) 6 hours, respectively. Production of TNF-α cytokine was measured by ELISA and mRNA level was analyzed by RT-PCR. Data shows mean ± SD values of duplicate determinations from three independent experiments. * and ** were calculated from comparing with LPS-stimulation value. The level of β-actin mRNA was compared in parallel to confirm the equivalency of the cDNA preparation. The RNA value in the cells stimulated by LPS without KIOM-MA treatment was set to 1.00 and the effect by treatment was quantitated using i-MAX Gel Image Analysis System (Core Bio, Seoul, Republic of Korea). The experiment was repeated three times.
