A Novel Herbal Medicine KIOM-MA Exerts an Anti-Inflammatory Effect in LPS-Stimulated RAW 264.7 Macrophage Cells
Figure 4
Effect of KIOM-MA on the expression of iNOS or COX-2 (a) protein and (b) mRNA in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were pretreated with KIOM-MA for 30 min before being incubated with LPS for 24 hours. Equal amounts of protein were separated by SDS-PAGE and immunoblotted with iNOS and COX-2 antibodies. Equal loading of protein was confirmed by β-actin. iNOS and COX-2 mRNAs were analyzed by RT-PCR in RAW 264.7 cells. The β-actin mRNA was used to confirm the equivalency of the cDNA preparation. The protein or mRNA value in the LPS-stimulated cells without KIOM-MA treatment was set to 1.00 and the effect by treatment was quantitated. The experiment was repeated three times.
