Research Article
A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach
Table 1
Proteins showing altered abundance after treatment with either AS extract or FA compared to the DMSO control and identified by LC-MS/MS.
| ID | Protein identity | Accession number | Abbreviation | MW/pI | Score | Match peptide | Sequence coverage (%) | | AS/DMSO | | FA/DMSO |
| 1 | Peroxiredoxin-6 | P30041 | PRDX6 | 25019/6.00 | 40 | 3 | 12 | ↑ | 1.91 | ↑ | 1.96 | 2 | Annexin A2 | P07355 | ANXA2 | 38580/7.57 | 110 | 3 | 9 | ↑ | 1.50 | ↑ | 1.51 | 3 | Peroxiredoxin-4 | Q13162 | PRDX4 | 30521/5.86 | 105 | 4 | 14 | ↑ | 2.02 | ↑ | 1.88 | 4 | Triosephosphate isomerase | P60174 | TPIS | 26713/6.45 | 236 | 7 | 27 | ↑ | 1.86 | ↑ | 1.52 | 5 | Parkinson disease protein 7 | Q99497 | PARK7 | 19878/6.33 | 67 | 2 | 12 | ↑ | 1.57 | ↑ | 1.77 | 6 | GTP-binding protein SAR1a | Q9NR31 | SAR1A | 22353/6.21 | 45 | 1 | 5 | ↑ | 1.72 | ↑ | 1.51 | 7 | Glutathione S-transferase P | P09211 | GSTP1 | 23341/5.43 | 214 | 7 | 43 | ↑ | 1.70 | ↑ | 1.50 | 8 | Glutathione S-transferase P | P09211 | GSTP1 | 23341/5.44 | 174 | 6 | 38 | ↑ | 2.54 | ↑ | 1.50 | 9 | Peroxiredoxin-2 | P32119 | PRDX2 | 21878/5.66 | 135 | 5 | 23 | ↑ | 1.77 | ↑ | 1.96 | 10 | Translationally controlled tumor protein | P13693 | TCTP | 195834.84 | 119 | 4 | 17 | ↑ | 1.57 | ↑ | 1.71 | 11 | Protein disulfide-isomerase A3 | P30101 | PDIA3 | 56747/5.98 | 1019 | 27 | 40 | ↓ | 0.53 | ↓ | 0.51 | 12 | Ubiquitin-conjugating enzyme E2 | P61088 | UBE2N | 17127/6.13 | 2.5 (criteria) | 4 | 26.3 | ↑ | 2.04 | ↑ | 1.77 | 13 | Nucleoside diphosphate kinase B | P22392 | NDKB | 17287/8.52 | 70 | 4 | 28 | ↓ | 0.63 | ↓ | 0.63 | 14 | Microtubule-associated protein RP/EB family member1 | Q15691 | MARE1 | 29980/5.02 | 117 | 4 | 20 | ↓ | 0.49 | ↓ | 0.62 | 15 | Protein SEC13 homolog | P55735 | SEC13 | 35518/5.22 | 130 | 4 | 9 | ↑ | 1.64 | ↑ | 1.60 | 16 | Serine-threonine kinase receptor-associated protein | Q9y3f4 | STRAP | 38414/4.98 | 2.5 (criteria) | 5 | 18.9 | ↑ | 1.97 | ↑ | 1.78 | 17 | Heat shock protein 60 | P10809 | HSP60 | 61016/5.70 | 379 | 15 | 19 | ↑ | 1.99 | ↑ | 1.56 | 18 | Isoform A of eukaryotic translation initiation factor 5A-1 | P63241-2 | IF5A1 | 20157/5.07 | 2.5 (criteria) | 4 | 21.2 | ↓ | 0.56 | | | 19 | L-Lactate dehydrogenase B chain | P07195 | LDHB | 36615/5.17 | 115 | 5 | 17 | ↑ | 2.01 | | | 20 | proteosome activator complex subunit 1 | Q06323 | PSME1 | 28705/5.78 | 289 | 7 | 27 | ↑ | 2.77 | | | 21 | Heat shock protein beta-1 | P04792 | HSPB1 | 22768/5.98 | 223 | 9 | 37 | ↑ | 1.65 | | | 22 | Phosphoglycerate kinase 1 | P00558 | PGK1 | 44586/8.30 | 94 | 1 | 4 | ↑ | 2.05 | | | 23 | Vimentin | P08670 | VIME | 53619/5.06 | 2.5 (criteria) | 10 | 23.8 | ↓ | 0.64 | | | 24 | Vimentin | P08670 | VIME | 53619/5.07 | 2.5 (criteria) | 10 | 23.8 | ↓ | 0.43 | | | 25 | Annexin A5 | P08758 | ANXA5 | 35914/4.94 | 272 | 13 | 40 | ↓ | 0.47 | | | 26 | Calpain small subunit 1 | P04632 | CAPNS1 | 28298/5.05 | 2.5 (criteria) | 8 | 36.2 | ↑ | 1.62 | | | 27 | Heat shock protein 6 | O14558 | HSP6 | 17125/5.95 | 75 | 4 | 38 | ↑ | 1.67 | | | 28 | Galectin-1 | P09382 | LEG1 | 14706/5.34 | 81 | 3 | 19 | ↓ | 0.66 | | | 29 | Synaptic vesicle membrane protein VAT-1 homolog | Q99536 | VAT1 | 41893/5.88 | 2.5 (criteria) | 8 | 28 | ↑ | 1.78 | | | 30 | Ferritin light chain | P02792 | FRIL | 20007/5.51 | 2.5 (criteria) | 3 | 20 | | | ↑ | 2.48 | 31 | Stathmin | P16949 | P08758 | 17292/5.76 | 134 | 6 | 36 | | | ↑ | 1.96 | 32 | Glutaredoxin | O76003 | GLRX3 | 37408/5.31 | 152 | 5 | 18 | | | ↑ | 1.66 | 33 | Actin-related protein 2/3 complex subunit 5 | O15511 | ARPC5 | 16310/5.47 | 179 | 6 | 27 | | | ↑ | 1.53 | 34 | Annexin A5 | P08758 | ANXA5 | 35914/4.94 | 2.5 (criteria) | 16 | 65 | | | ↑ | 1.53 |
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