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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 471312, 7 pages
http://dx.doi.org/10.1155/2012/471312
Research Article

Chemical Composition, Toxicity and Vasodilatation Effect of the Flowers Extract of Jasminum sambac (L.) Ait. “G. Duke of Tuscany”

1Department of Biochemistry, Faculty of Pharmacy, Mahidol University, 447 Sri Ayudthya, Rajathevi, Bangkok 10400, Thailand
2Pharmaceutical and Natural Products Department, Thailand Institute of Scientific and Technological Research (TISTR), Klong 5, Klong Luang, Pathum Thani 12120, Thailand

Received 11 October 2011; Accepted 1 January 2012

Academic Editor: Vincenzo De Feo

Copyright © 2012 Phanukit Kunhachan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Phytochemical analysis of the ethanolic Jasmine flower extract of Jasminum sambac (L.) Ait. “G. Duke of Tuscany” revealed the mixtures of coumarins, cardiac glycosides, essential oils, flavonoids, phenolics, saponins, and steroids. However, alkaloids, anthraquinones, and tannins were not detected. By intravenous injection at a single dose of 0.5 mL/mouse (15 mg) of the flower extract, no systemic biological toxicity demonstrated in ICR mice was observed. In Wistar rats, the LD50 of the extract was higher than 5,000 mg/kg BW by oral administration. Vasodilatation effect of the 95% ethanolic extract on isolated aortic rats was also investigated. Compared with the control group, the Jasmine flowers extract in 0.05% DMSO clearly reduced tonus of isolated endothelium thoracic aortic rings preconstricted with phenylephrine (  M), as a dose-dependent manner. Nevertheless, this pharmacological effect disappeared after the preincubation of the rings with atropine (  M) or with -nitro-L-arginine (  M). These are possibly due to the actions of the active components on the vessel muscarinic receptors or by causing the release of nitric oxide.