The ent-15𝛼-Acetoxykaur-16-en-19-oic Acid Relaxes Rat Artery Mesenteric Superior via Endothelium-Dependent and Endothelium-Independent Mechanisms

Table 1

Comparison of 𝐸_max and pD₂ values of KA-acetoxy against tonic contractions induced by phenylephrine in isolated rat mesenteric rings.

Phenylephrine 10 (𝜇M)	𝐸max	pD2
(condition)	(percentage of relaxation)	(value)
Endothelium intact	92.8 ± 3.7	6.0 ± 0.3
Endothelium denuded	95.2 ± 2.8	4.6 ± 0.2***
L-NAME (100 𝜇M)	67.3 ± 4.3***	4.1 ± 0.1***
Indomethacin (10 𝜇M)	61.2 ± 7.4***	4.5 ± 0.3*
L-NAME + indomethacin	66.9 ± 3.6***	4.7 ± 0.3*
Atropine (1 𝜇M)	73.6 ± 6.6*	4.4 ± 0.2***
ODQ (10 𝜇M)	77.7 ± 3.6*	4.6 ± 0.5*
Hydroxycobalamine (10 𝜇M)	68.1 ± 6.7**	4.6 ± 0.3*
L-arginine (1000 𝜇M)	91.4 ± 2.9	5.4 ± 0.4
L-NAME + L-arginine	100 ± 0	5.3 ± 0.4

Values are expressed as means ± SEM of six experiments. These experiments were performed in mesenteric rings with functional endothelium. *𝑃<0.05, **𝑃<0.01 and ***𝑃<0.001 versus endothelium intact. The data were analysed by one-way Anova followed by the Bonferroni post-test.