Effect of Mucuna pruriens Seed Extract Pretreatment on the Responses of Spontaneously Beating Rat Atria and Aortic Ring to Naja sputatrix (Javan Spitting Cobra) Venom
Figure 1
Effect of Naja sputatrix venom on the atrial rate (TOP, venom concentration (a) = 5 μg/mL, (b) = 50 μg/mL, and (c) = 250 μg/mL) and atrial contractility (BOTTOM, venom concentration (d) = 5 μg/mL, (e) = 50 μg/mL, and (f) = 250 μg/mL) of isolated atria from untreated (dotted line) and MPE-pretreated rats (solid line). The isolated atrial preparations were suspended in a 10 mL organ bath containing Krebs-Henseleit solution maintained at 37°C and aerated with 95% O2 and 5% CO2. The effect of the venom on the atrial rate and contractility was monitored for 15 min for each concentration. Values represent mean ± S.E.M. (). *, **, *** compared to the untreated animals at the corresponding time using unpaired Student’s -test. One way repeated measures ANOVA followed by Bonferroni/Dunnett T3 post-hoc test were used to determine the effect of time on the atrial rate and contractility responses for each venom concentration. The effect of time was only significant at 250 μg/mL in the untreated group, .
