Research Article

Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation

Figure 3

Caspase-3 mRNA expression in HT-29. Cells were treated with CLG and then incubated with for 24 h. After incubation, cells were harvested and used real-time RTPCR analysis. Normalization relative to GAPDH was performed. Results presented in bar graph are the means ± SD of three independent experiments. The statistical significance is expressed as *, .
490136.fig.003