Ginseng Berry Extract Prevents Atherogenesis via Anti-Inflammatory Action by Upregulating Phase II Gene Expression
Figure 4
KGBE inhibits inflammatory gene expression in LPS-stimulated RAW264.7 cells. RAW264.7 cells were pretreated with the indicated concentrations of KGBE for 30 min. The cells were stimulated with 100 ng/mL LPS for 8 h (RT-PCR) and 12 h (Western blotting and medium analysis). (a, d) Intracellular protein and mRNA levels of inflammatory cytokines and iNOS were determined by Western blot and RT-PCR analyses. (b, c) Levels of nitrite and PGE2 were determined in the culture media using Griess reagents and an ELISA kit, respectively. (e, f) Levels of TNF-α and IL-1β were determined in the culture media using ELISA kits. Data shown in graphs are the mean ± S.D. (). * and ** versus LPS.
