Anti-Inflammatory Activities of a Chinese Herbal Formula IBS-20 In Vitro and In Vivo
Figure 8
Effects of IBS-20 or the component herbs on barrier function of fully differentiated Caco-2 monolayer. 5 × 105 cells per well were seeded on a 12-well transwell plate with 12 mm insert and cultured for 10–14 days. The polarized epithelial cell monolayer was then treated with herbal extracts of IBS-20 (100 μg/mL) or the individual component herbs (20 μg/mL), Paeonia lactiflora Pall (Bai Shao, BS), Saposhnikovia divaricata Schischk (Fang Feng, FF), and Phellodendron amurense (Huang Bai, HB), added to both apical and basolateral sides in the presence or absence of IFNγ (20 ng/mL, added to the basolateral side). TEER was measured at 48 hours after treatment. * versus respective; ϕ versus VEH-IFNγ. Data shown in bar graphs are the mean ± s.e.m and representative of at least two independent experiments.
