Neuroprotective Effects of San-Huang-Xie-Xin-Tang in the MPP⁺/MPTP Models of Parkinson’s Disease In Vitro and In Vivo

Figure 2

Effects of SHXT on MPP⁺-induced changes of TH staining (a)–(e) and numbers of TH-positive neurons (f) in rat primary mesencephalic neurons. Primary mesencephalic neurons ((a), control) treated with SHXT (25–75 μg/mL) 1 h prior to MPP⁺ (100 μM) addition for 48 h were further confirmed by staining with anti-TH antibody (green). Numbers of TH-positive neurons were counted under a fluorescent microscope. Bars represent the mean ± S.E.M. from six independent experiments. ^##𝑃<0.01 versus control (without any treatment), *𝑃<0.05 versus MPP⁺ only. Scale bar = 50 μm.

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