The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis
Figure 6
Detection of collagen secretion, TGF-β expression, and other related cell signals. LR5 and LR20 treatments are able to significantly upregulate collagen secretion by fibroblasts in a dose-dependent manner (a). It was also found that the upregulation of collagen was concomitant with the overexpression of TGF-β (b). It was further confirmed that p-Erk and p-p38 were also upregulated in cells after LR5 and LR20 treatments (c). In contrast, the expression of p-Erk and p-p38 was downregulated in S100-treated fibroblasts. The ratios of p-Erk and p-p38 were normalized against GAPDH and then compared with DMSO using the Fujifilm Multigauge system. The experiments were carried out in triplicate and showed the same results. *; ***.
