The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis

Table 1

Proteins showing altered abundance after treatment with either the LR extracts or shikonin compared to the DMSO control and identified by LC-MS/MS.

ID	Protein identity	Accession; number	Abbreviation	MW/pI	Score	Match; peptide	Sequence; coverage (%)	LR 5/DMSO	LR 20/DMSO	S100/DMSO
1	Glutathione S-transferase P	P09211	GSTP1	23/5.43	224	8	46	↑ 2.26	↑ 2.66	↑ 1.61
2	Eukaryotic translation initiation factor 5A-1	P47942	IF5A1	17/5.08	58	4	23	↑ 1.17	↑ 1.30	↓ 0.96
3	Peroxiredoxin-2	P32119	PRDX2	22/5.38	143	6	23	↑ 1.65	↑ 1.84	↓ 0.89
4	Actin, cytoplasmic-1	P60711	ACTB	42/5.29	136	4	8	↓ 0.44	↓ 0.89	↓ 0.22
5	T-complex protein 1 subunit beta	P78371	TCPB	36/8.43	169	12	21	↓ 0.88	↓ 0.68	↓ 0.66
6	Glutaredoxin-3	O76003	GLRX3	37/5.31	81	8	19	↓ 0.61	↓ 0.69	↓ 0.83
7	Translationally controlled tumor protein	P13693	TCTP	20/4.84	119	4	17	↓ 0.66	↓ 0.40	↓ 0.39
8	Cofilin-1	P23528	COF1	18/8.22	113	7	24	↓ 1/∞	↑ 9.67	↓ 1/∞
9	Serum albumin	P02768	ALBU	69/5.92	119	2	3	↑ 1.24	↑ 1.78	↑ 1.04
10	Microtubule-associated protein RP/EB family member 1	Q15691	MARE1	30/5.02	65	3	17	↓ 0.85	↓ 0.61	↓ 0.61
11	Chloride intracellular channel protein 1	O00299	CLIC1	27/5.09	114	3	12	↓ 0.89	↓ 0.64	↓ 0.69
12	Superoxide dismutase	P00441	SODC	16/5.70	61	1	9	↑ 1.02	↑ 1.54	↓ 0.91
13	Nucleoside diphosphate kinase A	P15531	NME1	17/5.83	63	2	5	↑ 1.20	↑ 1.30	↓ 0.92
14	Actin-related protein 2/3 complex subunit 5	O15511	ARPC5	16310/5.47	179	6	27	↓ 0.51	↓ 0.97	↓ 0.49
15	Stathmin	P16949	STMN1	17292/5.76	134	6	36	↑ 2.54	↑ 1.98	↑ 1.99
16	1,2-Dihydroxy-3-keto-5-methylthiopentene dioxygenase	Q9bv57	ADI1	21484.6/	2.5 (criteria)	4	22	↑ 1.75	↑ 2.21	↑ 1.22
17	Galectin-1	P09382	LEG1	14706/5.34	81	3	19	↓ 0.39	↓ 0.55	↓ 0.28
18	Proteosome activator complex subunit 1	Q06323	PSME1	28705/5.78	289	7	27	↑ 3.12	↑ 2.64	↑ 2.37
19	Annexin A5	P08758	ANXA5	35914/4.94	2.5 (criteria)	16	65	↑ 2.31	↑ 1.68	↑ 1.98
20	Peroxiredoxin-4	Q13162	PRDX4	30521/5.86	105	4	14	↑ 2.81	↑ 2.27	↑ 2.51
21	Phosphoglycerate kinase 1	P00558	PGK1	44586/8.30	94	1	4	↓ 0.45	↓ 0.43	↓ 0.41
22	14-3-3 protein epsilon isoform	P62258	YWHAE	26504/4.76		n/a	n/a	↑ 1.86	↑ 1.31	↑ 1.58