Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 1

Research Article

Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1 -Induced NF-κB Activation in Canine Articular Chondrocytes

Figure 1

Effect of botanical extracts and IL-1β on the proliferation of chondrocytes in vitro. Serum-starved chondrocytes were exposed to IL-1β (10 ng/mL) for 48 h, botanical extracts (10 μg/mL) for 72 h, cotreated first with botanical extracts (10 μg/mL each) for 24 h, and then with IL-1β (10 ng/mL) for 48 h, treated with DMSO (as control) for 72 h or left untreated for 72 h. Cell viability was examined by MTT assay. The MTT assay is a spectrophotometric measurement of the cell viability as a function of the mitochondrial activity. This assay was performed in triplicate and the results are provided as mean values with standard deviations from three independent experiments. Treatments: Untreated control; IL-1β; RC (Rosa canina); SA (Salix alba); UD (Urtica dioica).

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.