Curcumin-Induced Apoptosis in Human Hepatocellular Carcinoma J5 Cells: Critical Role of Ca⁺²-Dependent Pathway

Figure 4

DNA damage and DNA fragmentation were induced by curcumin in J5 cells. Cells were incubated with 0, 25, and 50 μM curcumin for 24 h, and DNA damage was examined by (a) DAPI staining, (b) comet assay, and photographed by fluorescence microscope. (c) J5 cells were treated with 25 μM curcumin for 0, 12, 24, 36, and 48 h, and DNA fragmentation was determined with DNA gel electrophoresis.

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(b)

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