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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 515901, 11 pages
http://dx.doi.org/10.1155/2012/515901
Research Article

Flavonoids Isolated from Korea Citrus aurantium L. Induce G2/M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells

1Research Institute of Life science and College of Veterinary Medicine, Gyeongsang National University, Gazwa, Jinju 660-701, Republic of Korea
2Korea National Animal Research Resource Center and Korea National Animal Bio-resource Bank, Gyeongsang National University, Gazwa, Jinju 660-701, Republic of Korea
3Department of Nursing Science, International University of Korea, Jinju 660-759, Republic of Korea
4Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine and Gyeongnam Regional Cancer Center, Gyeongsang National University Hospital, Jinju 660-702, Republic of Korea
5Clinical Research Institute, Gyeongsang National University Hospital, Jinju 660-702, Republic of Korea

Received 5 August 2011; Accepted 14 September 2011

Academic Editor: Jae Youl Cho

Copyright © 2012 Do-Hoon Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim of the Study. Citrus species is used in traditional medicine as medicinal herb in several Asian countries including Korea. Flavonioids became known as various properties, such as anti-oxidants, anti-inflammation and anti-cancer, and so forth. The present study, the anti-cancer effect of flavonioids isolated from Citrus aurantium L. in human gastric cancer AGS cells has been investigated. Materials and Methods. The anti-proliferative activity was assayed using MTT assay. Cell cycle analysis was done using flow cytometry and apoptosis detection was done using by hoechst fluorescent staining and Annexin V-propidium iodide double staining. Western blot was used to detect the expression of protein related with cell cycle and apoptosis. Results. Flavonoids isolated from Citrus aurantium L. have the effect of anti proliferation on AGS cells with IC50 value of 99 μg/mL. Flavonoids inhibited cell cycle progression in the G2/M phase and decrease expression level of cyclin B1, cdc 2, cdc 25c. Flavonoids induced apoptosis through activate caspase and inactivate PARP. Conclusions. Flavonoids isolated from Citrus aurantium L. induced G2/M phase arrest through the modulation of cell cycle related proteins and apoptosis through activation caspase. These finding suggest flavonoids isolated from Citrus aurantium L. were useful agent for the chemoprevention of gastric cancer.