Flavonoids Isolated from Korea Citrus aurantium L. Induce G2/M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells

Figure 5

Flavonoids induce apoptosis of AGS cells. Flavonoids were added at the indicated concentrations, and cells were incubated for 24 h. (a) Hoechst 33258 staining of AGS cells treated with or without flavonoids for 24 h. Fragmented or condensed nuclei could be observed at 1000x magnification in the flavonoids-treated group as indicated by the arrows. (b) Apoptosis was assessed by Annexin V-PI double staining. (c) Statistical analysis of apoptosis rate. Each value represents the mean of three experiments; bars, ±SD. (*𝑃<0.05, **𝑃<0.01 versus control group.)

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