Scutellaria baicalensis Extracts and Flavonoids Protect Rat L6 Cells from Antimycin A-Induced Mitochondrial Dysfunction
Figure 3
S. baicalensis extracts prevented polarized MMP. Cells were treated with AMA alone for 24 h. (a) Cells were pretreated with baicalein, baicalin, wogonin (50 μg/mL each) for 1 h and with extracts (50, 100, 200 μg/mL each for 1 h), and then 100 μg/mL of AMA was added. Red fluorescence (excitation 550 nm, emission 600 nm) and green fluorescence (excitation 485 nm, emission 535 nm) were determined by using a fluorescence plate reader. (b) Confocal images show JC-1 fluorescence (60 × 2.5). Cells were pretreated with 50 μg/mL for 1, and then 100 μg/mL of AMA was added. Depolarized mitochondria were detected by green fluorescence, and polarized mitochondria were detected by red fluorescence. Data are expressed as a percentage of the control. *.
