Refined Qingkailing Protects MCAO Mice from Endoplasmic Reticulum Stress-Induced Apoptosis with a Broad Time Window

Figure 3

Effects of RQKL injection at different time points in mice undergoing middle cerebral artery occlusion. Flow chart of time window experiment of RQKL (A). Cerebral infarct volume (B, C) and neurological function scores (D, E) were evaluated 24 h after brain ischemia. Five brain coronal sections, 2 mm thick, were selected for tetrazolium chloride staining. Red stain represents normal tissues; white represents the infarct region (B). In panels (D) and (E), high scores represent serious injury. RQKL injection at 0, 1.5, 3, or 6 h after infarction significantly reduced infarct volume and improved focal and general neurological function, but 9 h group had no effects. ^ΔΔ𝑃<0.01, versus model group, ^#𝑃<0.05, ^##𝑃<0.01, versus model group. Data are expressed as mean ± SEM, the numbers of each group were 10, 11, 12, 9, 9, 12, 9, respectively.