Retinoic Acid Induces Apoptosis of Prostate Cancer DU145 Cells through Cdk5 Overactivation
Figure 4
Treatment of p35 cleavage inhibitor blocks RA-induced DU145 apoptosis. DU145 cells were treated as follows: control, RA (10 μM), RA + CP, CP (10 μM) for 4 days after 1-day serum-free pretreatment. Cell apoptosis was analyzed by sub-G1 distribution (a) and Annexin V staining (b) as described in Materials and Methods. Sub-G1 distribution was detected by flow cytometry with propidium iodide staining. Annexin V staining was performed by specific antibody with FITC conjugation. The apoptotic cells were counted per 100 cells in a random microscopic field. The independent experiments were repeated 3 times. Data are represented as means ± SEM; versus control group; versus RA group.
