Inhibition of Connexin 26/43 and Extracellular-Regulated Kinase Protein Plays a Critical Role in Melatonin Facilitated Gap Junctional Intercellular Communication in Hydrogen Peroxide-Treated HaCaT Keratinocyte Cells
Figure 2
Melatonin reduced ROS production and facilitated the decreased GJIC activity in H2O2-treated HaCaT cells. (a) Cells were exposed to H2O2 (300 μM) with or without melatonin (2 mM) for 24 h. ROS generation (%) was measured using ROS-sensitive fluorometric probe 2,7-dichlorofluorescein diacetate (DCFDA) by flow cytometric analysis. (b) Quantified graph for ROS production. Data represent means ± SD. ## versus untreated control. *** versus melatonin treated cells. (c) GJIC was assessed using the scrape-loading/dye-transfer (SL/DT) method under an inverted fluorescence microscope (100x). (d) Quantification of recovery rate.
