Inhibition of Connexin 26/43 and Extracellular-Regulated Kinase Protein Plays a Critical Role in Melatonin Facilitated Gap Junctional Intercellular Communication in Hydrogen Peroxide-Treated HaCaT Keratinocyte Cells

<table>Melatonin significantly decreased the phosphorylation of ERK alone, but not p38 MAPK or JNK in H<sub>2</sub>O<sub>2</sub>-treated HaCaT cells. Cells were exposed to H<sub>2</sub>O<sub>2</sub> (300 <i>μ</i>M) with or without melatonin (1 or 2 mM) for 24 h. (a) Western blotting was performed for phospho-ERK, ERK, phospho-p38, p38, phospho-JNK, and JNK. (b) Graphs represent relative level of phospho-ERK/ERK, phospho-p38/p38, and phospho-JNK/JNK. (c) Effect of ERK inhibitor PD98059 on GJIC using the SL/DT method. (d) Quantification of recovery rate.</table>

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Figure 4

Figure 4: Inhibition of Connexin 26/43 and Extracellular-Regulated Kinase Protein Plays a Critical Role in Melatonin Facilitated Gap Junctional Intercellular Communication in Hydrogen Peroxide-Treated HaCaT Keratinocyte Cells 

Figure 4 | Inhibition of Connexin 26/43 and Extracellular-Regulated Kinase Protein Plays a Critical Role in Melatonin Facilitated Gap Junctional Intercellular Communication in Hydrogen Peroxide-Treated HaCaT Keratinocyte Cells 