Terminalia catappa Exerts Antimetastatic Effects on Hepatocellular Carcinoma through Transcriptional Inhibition of Matrix Metalloproteinase-9 by Modulating NF-κB and AP-1 Activity
Figure 4
TCE suppresses MMP-9 expression at a transcriptional level. Huh7 cells were treated with TCE (0, 25, 50, 75, and 100 g/mL) for 24 h and then subjected to (a) reverse transcription-PCR and (b) quantitative real-time PCR to analyze the mRNA expression of MMP-9. (c) MMP-9 promoter reporter assay to analyze the promoter activity of MMP-9. Luciferase activity, determined in triplicates, was normalized to -galactosidase activity. The values represented the means SD of at least three independent experiments. * as compared with the vehicle group.
