From Omics to Drug Metabolism and High Content Screen of Natural Product in Zebrafish: A New Model for Discovery of Neuroactive Compound
Figure 3
Cell-cycle analysis of zebrafish embryos after treatment with different concentration of an antiangiogenesis compound named trans--trimethoxystilbene (TMS). Tg(fli-1:EGFP) zebrafish embryos treated with TMS for 20 h were then trypsinized and DRAQ5-stained for cell-cycle studies by flow cytometry. (a) Wildtype embryos did not show GFP-expressing-cells. (b) Tg(fli-1:EGFP) showed GFP expressing and non-GFP expressing cells. (c) 10 μM TMS and (d) 30 μM TMS exerted G2/M cell-cycle arrest preferentially in endothelial cells.
