From Omics to Drug Metabolism and High Content Screen of Natural Product in Zebrafish: A New Model for Discovery of Neuroactive Compound

Figure 5

MPTP induces DA neuron loss in zebrafish. (a) Representative picture of anti-TH whole mount immunostaining. TH⁺ neurons in diencephalic region were indicated by bracket, dorsal view. L-dep, L-deprenyl (selegiline), a selective MAO-B inhibitor, was used as positive control. (b) Counting of TH⁺ neuron. (c) Relative fold change of th gene expression as compared to control, MPTP downregulated th gene expression. ^#𝑃<0.05 and ^##𝑃<0.01 compared with untreated control. ^∗𝑃<0.05 and ^∗∗𝑃<0.01 compared with MPTP treated alone.

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