From Omics to Drug Metabolism and High Content Screen of Natural Product in Zebrafish: A New Model for Discovery of Neuroactive Compound

Table 2

Potential marker genes for PD.

Gene	Function	Assessment method	Reference
Tyrosine hydroxylase (TH)	Catalytic conversion of the amino acid L-tyrosine to dihydroxyphenylalanine	Immunostaining, locomotion behaviour test	[28, 64, 66]
Dopamine transporter (DAT)	Membrane-spanning protein for pumping neurotransmitter DA back into cytosol from the synaptic region	Whole mount in situ hybridization (WISH), swimming behaviour	[65]
Vesicular monoamine transporter 2 (VMAT2)	Integral membrane protein for transporting neurotransmitter carrying monoamine structure, for example, dopamine and norepinephrine from cellular cytosol into synaptic vesicles	Visualization in VMAT2: GFP transgenic fish	[67]
MAO-B	Catalytic oxidation of monoamines	Monoamine oxidase enzyme histochemistry	[66]
PARKIN (PARK2)	Gene knockdown leads to complex I deficiency and dopaminergic neuronal cell loss	WISH, whole-mount antibody immunofluorescence, behaviour analysis	[68]
LRRK2	Genetic mutant caused loss of DA neuron and locomotive defect	WISH, swimming behaviour	[69]