Polygonum cuspidatum Extract Induces Anoikis in Hepatocarcinoma Cells Associated with Generation of Reactive Oxygen Species and Downregulation of Focal Adhesion Kinase

Figure 7

The relation between PCE-induced ROS and FAK downregulation. (a) Bel-7402 cells were transfected with recombinant human FAK and empty vector and subjected to suspension culture, PCE (200 μg/mL) treatment, and ROS assay. (b) NAC pretreated or untreated Bel-7402 cells were treated with PCE (200 μg/mL) and subjected to western blot. **𝑃<0.01, versus control group; ^∆𝑃>0.05, re-FAK group versus PCE-treated empty vector group.

(a)

(b)