Bambusae caulis in Liquamen Suppresses the Expression of Thymus and Activation-Regulated Chemokine and Macrophage-Derived Chemokine in Human Keratinocytes due to Antioxidant Effect
BCL suppressed the activation of NF-κB via IκB but not via p38 pathway. The phosphorylation of p38 MAPK induced after incubation with IFN-γ was not affected by BCL (2%) treatment. Phosphorylation of p38 MAPK was significantly suppressed by SB203580. (b) IFN-γ significantly increased the nuclear translocation of NF-κB p65, but BCL (2%) decreased the nuclear levels of NF-κB p65 by about 60% in IFN-γ-stimulated HaCaT cells. NF-κB inhibitor, Bay11-7082, suppressed IFN-γ-induced nuclear translocation of NF-κB by about 85%. (c) The inhibitory effect of BCL on IFN-γ-induced nuclear translocation of NF-κB p65 was observed using immunocytochemistry analysis. (d) BCL (2%) markedly blocked IFN-γ- (10 ng/mL)induced degradation of IκB-α. (e) IFN-γ-induced phosphorylation of IκB-α was also significantly suppressed by the addition of 2% BCL. All of data are presented as mean ± SEM of three separate experiments. *, **, and *** versus IFN-γ alone.
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