Oleifolioside A, a New Active Compound, Attenuates LPS-Stimulated iNOS and COX-2 Expression through the Downregulation of NF-κB and MAPK Activities in RAW 264.7 Macrophages
Figure 1
Effects of oleifoliosides A on LPS-stimulated cell viability and NO, PGE2 production in RAW 264.7 macrophages. (a) Chemical structure of oleifoliosides A. (b) Cells were treated with the concentrations of oleifoliosides A (0, 5, 10, 15, 20, 30, or 40 μM) 1 h before LPS (500 ng/mL) presence or absence for 24 h. Cell viability was assessed by MTT assays. (c) Cells were treated with the indicated concentrations of oleifoliosides A (0, 5, 10, or 15 μM) and NIL (20 μM) 1 h before LPS (500 ng/mL) treatment for 24 h, the nitrite production was measured by the Griess reaction. (d) Cells were treated with the indicated concentrations of oleifoliosides A and NS-398 (20 μM) 1 h before LPS (500 ng/mL) treatment for 24 h, the PGE2 concentration was determined by ELISA kit. The values are presented as mean ± S.E. of three independent experiments. per experiment. versus LPS and versus LPS.
