Effects of Different Electroacupuncture Scheduling Regimens on Murine Bone Tumor-Induced Hyperalgesia: Sex Differences and Role of Inflammation
Figure 5
Fluorescent immunohistochemistry and H&E staining for neutrophils (a)–(d) or macrophages (e)-(f) in peripheral tumor hind paw tissue. All images were magnified to 40X with a Nikon 80i fluorescent microscope and captured with ACT-1 software (Nikon, Melville, NY). White arrows (b), (f) indicate labeled immunocytes; black arrows (d) indicate neutrophils stained with H&E. EA-2X/3 significantly reduced the number of neutrophils at the tumor site ((b) compared to (a)), but produced no change in the numbers of macrophages ((e) versus (f)). Scale bars represent 20 μm.
