Inchingorei-san (TJ-117) and Artemisiae Capillaris Herba Induced Prolonged Survival of Fully Mismatched Cardiac Allografts and Generated Regulatory Cells in Mice
Figure 2
Evidence of generation of regulatory cells in CBA allograft recipients treated with Artemisiae Capillaris Herba (ACH). (a)–(c) Cardiac allograft survival after adoptive transfer of whole splenocytes, CD4+, or CD4+CD25+ cells. MST, median survival time; #P < 0.05 and *P < 0.01 for difference between 2 groups. (d) CD4, CD25, and Foxp3 expression in splenocytes, as determined by flow cytometry. Data are mean ± SD ( mice in each group) for the percentage of CD4+CD25+Foxp3+ in CD4+ cells. *P < 0.01 for difference between 2 groups. (e) Foxp3+ cells with use of double immunostaining of cardiac allografts obtained 30 days after transplantation from treatment with ACH and untreated recipients (magnification ×40, resp.).
