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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 703034, 12 pages
Research Article

Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2/M Phase Arrest

1Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, China
2College of Life Sciences, Liaoning Normal University, Dalian 116029, China
3Second Clinical Hospital, Jilin University, Changchun 130041, China

Received 14 June 2011; Revised 8 September 2011; Accepted 15 September 2011

Academic Editor: Alfred Längler

Copyright © 2012 Muhammad Khan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Artemisia argyi is a widely used medicinal plant in China. The present study was designed to identify the bioactive constituents with antiglioma activity from leaves of Artemesia argyi. A bioactivity guided approach based on MTT assay for cells growth inhibition led to the isolation of a flavonoid, “jaceosidin” from ethanol extract of leaves of Artemesia argyi. The growth inhibitory effect of jaceosidin was explored using flow cytometry and Western blot studies. Our results showed that jaceosidin exerts growth inhibitory effect by arresting the cells at G2/M phase and induction of apoptosis. Furthermore, our study revealed that induction of apoptosis was associated with cell cycle arrest at G2/M phase, upregulation of p53 and Bax, decrease in mitochondrial membrane potential, release of cytochrome c, and activation of caspase 3. This mitochondrial-caspase-3-dependent apoptosis pathway was confirmed by pretreatment with caspase 3 inhibitor, Ac-DEVD-CHO. Our findings suggested that jaceosidin induces mitochondrial-caspase-3-dependent apoptosis in U87 cells by arresting the cell cycle at G2/M phase.