Phenethyl Isothiocyanate (PEITC) Inhibits the Growth of Human Oral Squamous Carcinoma HSC-3 Cells through G₀/G₁ Phase Arrest and Mitochondria-Mediated Apoptotic Cell Death

Figure 4

PEITC affected the reactive oxygen species (ROS) productions, intracellular Ca²⁺ release, and the levels of mitochondrial membrane potential (ΔΨ_𝑚) in HSC-3 cells. Cells were treated with 2.5 μM of PEITC for 0, 3, 6, 9, 12, 24, and 48 h for the production of ROS (a) and Ca²⁺ (b), or incubation for 0, 3, 6, 12, 24, and 48 h in the changes in ΔΨ_𝑚 (c). All samples were analyzed by flow cytometric assay as described in Section 2. Data are presented as the mean ± S.E.M. of three independent experiments. *𝑃<0.05, significantly different compared with PEITC-treatment.

(a)

(b)

(c)