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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 728196, 8 pages
http://dx.doi.org/10.1155/2012/728196
Research Article

Zuonin B Inhibits Lipopolysaccharide-Induced Inflammation via Downregulation of the ERK1/2 and JNK Pathways in RAW264.7 Macrophages

1Immune Modulator Research Center, Korea Research Institute of Bioscience and Biotechnology, 685-1 Yangchung-ri, Ochang-eup, Cheongwon-gun, Chungbuk, Republic of Korea
2Herbal Medicine, Evidance Based Medicine Research Center, Korea Institute of Oriental Medicine, Exporo 483, Yusung, Daejeon 305-811, Republic of Korea

Received 25 July 2011; Revised 19 October 2011; Accepted 24 October 2011

Academic Editor: Olumayokun A. Olajide

Copyright © 2012 Mee-Young Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated whether Zuonin B exerts immunological effects on RAW264.7 cells. Zuonin B, isolated from flower buds of Daphne genkwa, suppressed the levels of nitric oxide and prostaglandin E2, as well as proinflammatory cytokines, such as tumor necrosis factor-α and interleukin-(IL-) 6, in lipopolysaccharide-stimulated macrophages. Moreover, the compound inhibited cyclooxygenase-2 and inducible nitric oxide synthase expression. Zuonin B attenuated NF-kappaB (NF-κB) activation via suppressing proteolysis of inhibitor kappa B-alpha (IκB-α) and p65 nuclear translocation as well as phosphorylation of extracellular signal-regulated kinase 1/2 and c-Jun N-terminal kinase. Additionally, IL-4 and IL-13 production in ConA-induced splenocytes was inhibited by Zuonin B. In conclusion, the anti-inflammatory effects of Zuonin B are attributable to the suppression of proinflammatory cytokines and mediators via blockage of NF-κB and AP-1 activation. Based on these findings, we propose that Zuonin B is potentially an effective functional chemical candidate for the prevention of inflammatory diseases.