Zuonin B Inhibits Lipopolysaccharide-Induced Inflammation via Downregulation of the ERK1/2 and JNK Pathways in RAW264.7 Macrophages
Figure 4
Effect of Zuonin B on Th1 (TNF-α and IL-6) and Th2 (IL-4 and IL-13) type cytokine production in LPS-stimulated RAW264.7 cells and ConA-induced splenocytes. RAW264.7 cells were pretreated with different concentrations of Zuonin B for 1 h and stimulated with LPS (1 μg/mL) for another 24 h. Following incubation, the amounts of TNF-α (a) and IL-6 (b) released were determined with a TNF-α and IL-6 antibody-coated ELISA kit, as described in Section 2. Data represent the mean values of three experiments ± SEM. NC: untreated control cells; LPS: LPS only treatment.*Significant difference from NC and #significant difference from LPS: . Con-A-stimulated splenocytes were cultured in the presence or absence of Zuonin B. Cytokine levels were measured using ELISA. Cytokine levels, IL-4 (c) and IL-13 (d). NC: untreated control cells; ConA, ConA (1 μg/mL) only treatment. Data represent the mean values of three experimentsSEM. *Significant difference from NC and #significant difference from ConA, .
